• Theoretical analyses and experimental validation of effects caused by fluorinated substituent modification of DNA (Scientific Reports, 2020, in press)

    In collaboration with Osaka University Graduate School of Medicine, Department of Gastroenterology (Profs Yuichiro Doki and Masaki Mori) and Graduate School of Pharmaceutical Sciences (Prof Obika), as well as Taiho Pharmaceutical Co., Ltd, Koseki et al. performed the structural based studies of nucleotide medicine. Given that halogen-modified nucleic acid molecules, such as trifluorothymidine (FTD) and 5-fluorouracil, are widely used in medical science and clinical site, our study focused on the reason why a large differences between FTD and 5-fluorouracil occurs. The results showed the important structural changes caused by trifluoromethyl group, for the anti-tumor effect of FTD.

  • One-carbon metabolism for cancer diagnostic and therapeutic approaches (Cancer Letters, 2019)

    In the international collaboration with Japan (Professor Taniguchi) and Italy (Professor Vecchione), Dr. Asai demonstrated the significance of one-carbon metabolism in cancer and discussed the perspectives in precision medicine. Understanding 1C metabolism aids the development of novel cancer diagnostic and therapeutic approaches.

  • CD44/CD133-positive colorectal cancer stem cells are sensitive to trifluridine (FTD) exposure (Scientific Reports, 2019, in press).
    In collaboration with Taiho Pharmaceutical Co., Ltd, as well as Osaka University (Profs Yuichiro Doki and Masaki Mori), Tsunekuni et al. studied the effects of FTD exposure to CD44/CD133-positive cancer stem cells (CSCs) and elucidated firstly the mechanism on CSC-targeting FTD-containing chemotherapy for colorectal cancer.
  • Distinct methylation levels of mature microRNAs in gastrointestinal cancers (Nature Communications, 2019, in press).
    In collaboration with Osaka University (Profs Yuichiro Doki and Masaki Mori, and Associate Prof Hidetoshi Eguchi), Konno et al. have studied the methylation events in miRNA by utilizing the MS-based new technology and have found that the epitranscriptome information is critical for the accurate diagnosis of gastrointestinal cancers, suggesting that the findings provide a basis for new diagnostic strategies for early stage cancer and add a new dimension to our understanding of miRNA biology.
  • Reproducibility of CRISPR-Cas9 Methods for generation of conditional mouse alleles: a multi-center evaluation (Genome Biology, 2019, in press)

    In collaboration with the international consortium, the efficiency and conditions were studied and elucidated for further improvement of genetic engineering technology in animal model.

  • Computational analyses for cancer biology based on exhaustive experimental backgrounds (Cancer Drug Resistance, BOOK section, 2019)

    In the view of recent advance of the field of cancer science, Koseki et al. updated the interdisciplinary knowledge and information regarding the cancer metabolism, which may be involved in causing resistance to chemotherapy for further development of precision medicine.

  • Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice (Oncotarget, 2019).

    In collaboration with Osaka University (Prof Yuichiro Doki), Ueda et al. studied the mechanism of usefulness of Caenorhabditis elegans in the detection of cancer by urine. The genetically-engineered mice were used in the present study, which will facilitate further studies on mechanism of cancer detection by liquid biopsy in human.

  • A gemcitabine prodrug for selective treatment of pancreatic ductal adenocarcinoma (ChemMedChem, 2019, in press)

    In the collaboration with the Graduate School of Pharmaceutical Sciences, Osaka University (Prof. Satoshi Obika), Matsushita, et al. developed a gemcitabine prodrug. Given that the major concern in the use of anticancer chemotherapeutic drugs is host toxicity, this study will contribute to improve the treatment of pancreatic ductal adenocarcinoma.

  • Significant epitranscriptomes in heterogeneous cancer (Cancer Sci, 2019, in press)

    The significance of cancer RNA modification is updated and discussed and the implication is emerged in In this review article, by referring previous reports. The recent progress of epitranscriptomes (ETR) detection methods, and biological findings regarding the significance of ETR is emerged in cancer precision medicine.

  • Direct analysis of incorporation of an anticancer drug into DNA at single-molecule resolution (Scientific Reports, 2019).

    Ohshiro et al. published the novel methods and technology to study sequencing of DNA, in which trifluridine (FTD) is incorporated, at single-molecule resolution.