• Mitochondrial pyruvate carrier 1 expression controls cancer EMT and radioresistance (Cancer Sci, 2019) 
    In the collaboration with the Department of Radiation Oncology, Osaka University (Prof. Kazuhiko Ogawa), Takaoka, et al. investigated the mitochondrial pyruvate carriers (MPCs), key molecules in glycolysis and oxidative phosphorylation, and showed that they played critical roles in cancer metastasis. The phenomenon of epithelial-mesenchymal transition (EMT) was evaluated in the MPC-modified cells. The present study demonstrate that the MPCs are involved in the malignant behavior of epithelial cancer as therapeutic targets.
  • Disruption of endolysosomal pathway efficiently eliminates colorectal cancer stem cells (Cancer Research, 2019).
    Takeda and Haraguchi published the novel discovery of colorectal cancer stem cells, as a collaboration.
  • Semaphorin signaling via MICAL3 induces symmetric cell division to expand breast cancer stem-like cells (PNAS, 2019).

    Tomonaga and Gotoh at Kanazawa University published the novel discovery of Semaphorin signaling in the study of breast cancer stem cells, as a collaboration.

  • AI for medical use (Oncotarget, 2019)
    Konno and Ishii have updated the AI issues for medical use. The recent progress and reports indicate the potential of the medical diagnosis and treatment using AI, which will be recognized in the near future.  
  • Drug discovery of anticancer drugs targeting methylenetetrahydrofolate dehydrogenase 2 (Heliyon, in press)
    In collaboration with Osaka University (Profs Masaki Mori and Yuichiro Doki) and Kanazawa University (Prof Noriko Gotoh), Asai et al. demonstrated that methylenetetrahydrofolate dehydrogenase 2 (MTHFD2), an enzyme in one carbon metabolism, played a role in cancer cell metabolism and showed several candidate tool compounds for drug discovery. The findings of the present study will help to develop anticancer drugs targeting MTHFD2, with a view to minimizing the adverse effects of anticancer drugs.
  • Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells (Cell Death Discovery, 2018, in press)
    In collaboration with the Department of Radiation Oncology, Osaka University (Prof. Kazuhiko Ogawa), Tamari et al. revealed details of metabolic pathways that drive epigenetic control of cancer cell stemness and determine effective therapeutic targets in cancer stem cells (CSCs). The study indicated that intracellular polyamines inhibited the activity of histone lysine 4 demethylase enzymes, including lysine-specific demethylase-1 (LSD1), and demonstrated the significance in drug discovery.
  • A convolutional neural network uses microscopic images to differentiate between mouse and human cell lines and their radioresistant clones (Cancer Research, 2018, in press)

    In collaboration with the Department of Radiation Oncology, Osaka University (Prof. Kazuhiko Ogawa), Toratani et al. demonstrated that the utility of image recognition using the artificial intelligence (AI) technology for predicting minute differences among phase-contrast microscopic images of cancer cells and their radioresistant clones. This study demonstrates rapid and accurate identification of radioresistant tumor cells in culture using artifical intelligence, and this should have applications in future pre-clinical cancer research.

  • Block copolymer conjugate targets hepatocellular carcinoma stem cells (Oncogene, 2018)

    In collaboration with the Department of Surgery, Osaka University (Profs. Masaki Mori and Yuichiro Doki) and Tokyo Institute of Technology (Prof. Nobuhiro Nishiyama), Toshiyama et al. developed a block copolymer conjugate to increase the efficacy of reagents in cancer stem cells. The exposure increased the intracellular ROS concentration by inhibiting enzyme activity, permitting the induction of apoptosis and attenuation of cancer cell growth. This study demonstrated that it exerts a potent antitumor effect.

  • Computational trans-omics approach characterised methylomic and transcriptomic involvements and identified novel therapeutic targets for chemoresistance in gastrointestinal cancer stem cells (Scientific Reports, 2018, in press)

    In collaboration with the Department of Surgery, Osaka University (Profs. Masaki Mori and Yuichiro Doki), Konno et al. demonstrated by the computational trans-omics approach that the novel therapeutic targets are involved in the chemoresistance in gastrointestinal cancer stem cells. This mathematical method can be used to simultaneously quantify and identify chemoresistant potential targets in gastrointestinal cancer stem cells.

  • Enzymes of the one-carbon folate metabolism as anticancer targets predicted by survival rate analysis (Scientific Reports, 2018, in press)

    In collaboration with the Department of Surgery, Osaka University (Profs. Masaki Mori and Yuichiro Doki), Koseki et al. studied the significance of mitochondrial metabolism in cancer cells by computational approach as well as wet experiments, and demonstrated the importance of the one-carbon folate metabolism in metabolic cycle of tetrahydrofolate in mitochondria, SHMT2, MTHFD2, and ALDH1L2, suggesting the rationale of drug discovery in cancer medicine.